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URL: http://www.addgene.org/248333
Proper Citation: RRID:Addgene_248333
Insert Name: gRNAscaffold-tRNA
Organism: P. patens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Golden Gate fragments are amplified by PCR, with primers designed to produce half of each gRNA followed by BsaI recognition sites. Complete gRNA sequences to be reconstituted upon BsaI digestion and ligation during the Golgen Gate reaction.
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Source: Addgene